Further Evaluation of the Base Stability of Hydrophilic Interaction Chromatography Columns Packed with Silica or Ethylene-Bridged Hybrid Particles

One of the fundamental attributes a liquid chromatography column is its stability when exposed to acidic and basic mobile phases. However, there have been relatively few reports date on hydrophilic interaction (HILIC) columns. Here, we report results evaluations carried out for HILIC columns packed with ethylene-bridged hybrid or silica particles using accelerated conditions, employing 100% aqueous pH 11.3 ammonium bicarbonate phase at 70 °C. Under these primary mode failure was loss efficiency due formation voids resulting from hydrolysis particles. We investigated dependence surface area both unbonded sulfobetaine-bonded stationary The show clear trend increasing as decreases. Several commercially available that are recommended use high-pH phases were also evaluated. times 50% initial ranging 0.3 9.9 h. Columns containing unbonded, diol-bonded had longer lifetimes than amino-bonded sulfobetaine-bonded, hybrid-coated, superficially porous